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mouse tnf α paired antibodies  (R&D Systems)


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    Structured Review

    R&D Systems mouse tnf α paired antibodies
    Scatter plots of the association <t>between</t> <t>TNF-α</t> production and the IC 50 values (LDA and LMA) Scatter plots showing the association <t>between</t> <t>TNF-α</t> production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.
    Mouse Tnf α Paired Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+duoset/Mouse+TNF-alpha+DuoSet+ELISA/pmc13101707-56-32-36
    Average 97 stars, based on 1183 article reviews
    mouse tnf α paired antibodies - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections"

    Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

    Journal: International Journal for Parasitology: Drugs and Drug Resistance

    doi: 10.1016/j.ijpddr.2026.100642

    Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.
    Figure Legend Snippet: Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.

    Techniques Used: Control, Migration

    Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).
    Figure Legend Snippet: Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).

    Techniques Used: Activity Assay, Control

    Related Articles

    Concentration Assay:

    Article Title: PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking
    Article Snippet: ISD and poly(I:C) were incubated with Lipofectamine 2000 (Life Technologies) at a ratio of 1:1 in Opti-MEM (Life Technologies). .. The IL-6 and CXCL10 concentration in the culture supernatants was measured by a mouse Duoset (R&D Systems) according to the manufacturer's instructions. ..

    Article Title: PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking
    Article Snippet: ISD and poly I:C were incubated with Lipofectamine 2000 (Life Technologies) at a ratio of 1:1 in OptiMEM (Life technologies). .. The IL-6 and CXCL10 concentration in the culture supernatants was measured by a mouse Duoset (R&D Systems) according to the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Caveolin-1 gene therapy inhibits inflammasome activation to protect from bleomycin-induced pulmonary fibrosis
    Article Snippet: Cells from the BAL were stained with Diff-Quik TM (Siemens, Newark, DE) after cytospin. .. IL-1β levels in cells, mouse BAL, and lung homogenates were measured by ELISA according to the manufacturer’s instructions (Mouse DuoSet, R&D Systems, Minneapolis, MN). .. Total RNA was isolated from mouse or human lung tissue samples using Trizol reagent (Invitrogen, Carlsbad, CA). cDNA was prepared using Reverse Transcription System (Promega, Madison, WI) following the manufacturer’s instructions and quantitative RT-PCR was performed as previously described .

    Article Title: Discovery of a Potent and Selective Inhibitor of Human NLRP3 with a Novel Binding Modality and Mechanism of Action
    Article Snippet: After 30 min incubation 5 μM Nigericin (Adipogen) was added for 1 hr. .. Cell free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Article Title: Caveolin-1 gene therapy inhibits inflammasome activation to protect from bleomycin-induced pulmonary fibrosis.
    Article Snippet: .. IL-1β levels in cells, mouse BAL, and lung homogenates were measured by ELISA according to the manufacturer’s instructions (Mouse DuoSet, R&D Systems, Minneapolis, MN). ..

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action.
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Lactate Dehydrogenase Assay:

    Article Title: Discovery of a Potent and Selective Inhibitor of Human NLRP3 with a Novel Binding Modality and Mechanism of Action
    Article Snippet: After 30 min incubation 5 μM Nigericin (Adipogen) was added for 1 hr. .. Cell free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action.
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Incubation:

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..

    Article Title: Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action.
    Article Snippet: .. After 30-min incubation, 5 μM nigericin (Adipogen) was added for 1 h. Cell-free supernatants were removed and assessed by LDH assay (Roche) and ELISA for IL-1β according to the manufacturer’s instructions (mouse DuoSet, R&D Systems). ..



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    Scatter plots of the association <t>between</t> <t>TNF-α</t> production and the IC 50 values (LDA and LMA) Scatter plots showing the association <t>between</t> <t>TNF-α</t> production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.
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    Image Search Results


    Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.

    Journal: International Journal for Parasitology: Drugs and Drug Resistance

    Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

    doi: 10.1016/j.ijpddr.2026.100642

    Figure Lengend Snippet: Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.

    Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

    Techniques: Control, Migration

    Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).

    Journal: International Journal for Parasitology: Drugs and Drug Resistance

    Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

    doi: 10.1016/j.ijpddr.2026.100642

    Figure Lengend Snippet: Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).

    Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

    Techniques: Activity Assay, Control